T lymphocytes to chemokines

Two subpopulations of human T lymphocytes expressing different antigen receptors, § / g and + / ˇ , emigrate into inflamed tissues in distinctive patterns. We compared the transmigration of § / g and + / ˇ T cells to C-C and C-X-C chemokines using an in vitro transendothelial chemotaxis assay. The C-C chemokines monocyte chemoattractant protein (MCP)-1, RANTES, macrophage inflammatory protein (MIP)-1 § and MIP-1 g stimulated similar, dosedependent chemotaxis of purified + / ˇ T cells, whereas MCP-1, RANTES, and MIP-1 § produced greater chemotaxis of purified § / g T cells than MIP-1 g . In contrast, the C-X-C chemokines interleukin (IL)-8 and interferon+ inducible protein-10 (IP-10) did not promote chemotaxis of either § / g or + / ˇ T cells. Three + / ˇ T cell clones with differing CD4 and CD8 phenotypes also migrated exclusively to C-C chemokines. Phenotypic analysis of mononuclear cells that transmigrated from an input population of unfractionated peripheral blood mononuclear cells confirmed the results with purified + / ˇ T cells. Our data demonstrate that human peripheral blood § / g and + / ˇ T cells can transmigrate to MCP-1, RANTES, MIP-1 § , and MIP-1 g , and suggest that both T lymphocyte subpopulations share the capacity to emigrate in response to C-C chemokines during inflammation.